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发表于 2008-2-6 08:05 AM
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肿瘤生长或生存必需基因
最近发现(附录一),肿瘤的生长或生存存在必需基因
附录一 Growth and Survival—The Complete Toolkit
As tumors progress to a more aggressive state, they acquire multiple genetic alterations, some that have little functional impact and others that are essential for the continued growth and survival of the tumor cells. Schlabach et al. and Silva et al. have developed a functional genomics strategy that will allow, at a genome-wide level, systematic identification of genes required for cell growth and survival. Cell lines derived from human mammary and colorectal cancers and normal mammary tissue showed a similar pattern of so-called "essential" genes, with many residing within functional pathways known to be critical for fundamental cellular processes such as cell cycle and translational control. Importantly, however, additional genes were identified as being essential for the growth of specific cell lines. This functional genomics strategy complements the cancer genome sequencing approaches that have shown recent success and could set the stage for high-throughput discovery of cancer drugs.
Schlabach MR, Luo J, Solimini NL, Hu G, Xu Q, Li MZ, Zhao Z, Smogorzewska A, Sowa ME, Ang XL, Westbrook TF, Liang AC, Chang K, Hackett JA, Harper JW, Hannon GJ, Elledge SJ. 2008. Cancer proliferation gene discovery through functional genomics. Science. 2008 Feb 1;319(5863):620-4.
Silva JM, Marran K, Parker JS, Silva J, Golding M, Schlabach MR, Elledge SJ, Hannon GJ, Chang K. 2008. Profiling essential genes in human mammary cells by multiplex RNAi screening. Science. 2008 Feb 1;319(5863):617-20. |
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